Not known Facts About attune flow cytometer

Not known Facts About attune flow cytometer

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Data-enriched characterization of cell populations. Morphological info from pictures can incorporate for the richness of flow cytometry knowledge. Such as, the determine reveals an or else conventional apoptosis assay working with Annexin V and PI, adding mobile imaging to characterize cells in Each individual inhabitants to expose morphologically distinctive capabilities. These insights couldn't have been attained from multiplex staining by yourself.

Irrespective of whether you’re a newbie or a specialist, examine our methods to progress your analysis and experience in flow cytometry.

Combine acoustic focusing engineering with high-parameter Investigation for flexible spectral unmixing and conventional workflows.

Explore new insights into your experimental knowledge by visualizing populations using blended fluorescence and morphology information. Our software package interface wizards empower downsampling, concatenation (merged data files) and dimensionality applications which are crafted into the application for an intuitive, streamlined details Investigation expertise.

*Pricing is indicative only which is matter to vary. Remember to Make contact with your Agilent agent with any concerns. The knowledge set forth herein just isn't an offer or a commitment, each of that are matter to credit approval, stipulations, and availability with Agilent. Agilent Economic Methods is available in selected international locations.

Also, it is illuminating to find out the number of doublets slip via traditional singlet gates, and wonderful to verify the status of unexpected double-optimistic situations in my analysis."

Optimizing gating tactic. Even sturdy handbook singlet gating is mistake-vulnerable and remains a subjective choice stage in Practically all flow cytometry assays. Imaging can be employed to substantiate and modify gates to incorporate only single cells of interest.

Constant image high-quality even at superior throughput Acoustic focusing and a substantial-pace digital camera Incorporate to graphic these T cells continually at low or superior flow rates. Conveniently regulate concentrate and digital camera options to meet experimental needs.

Early indications of this contamination experienced initially been dismissed as debris. Backgating to find out new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also lets you use morphological options to discover appealing subpopulations that would not be evident from flow cytometry data by itself.

Perhaps most significantly, these activities contain cells of clearly unique phenotypes which may lead to incorrect conclusions concerning double constructive occasions (specifically in unusual populations).

Sound waves empower specific cell alignment, leading to Fantastic info excellent and ~10X faster sample flow rates.

Software note: Automatic graphic analysis cuts down person-to-consumer variability in flow cytometry gating approaches

Created-in dimensionality reduction applications amplified resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to substantiate predicted inhabitants resolution in lysed complete human blood. Person can watch situations from each cluster for visual affirmation of cell phenotypes.

Adaptable payment strategy from Agilent Economic Alternatives enable you to receive the crucial instrumentation you need for fast job, or to increase your lab.

At the center of each Attune Flow Cytometer lies our groundbreaking acoustic click here concentrating engineering, which makes use of audio waves to meticulously align cells within the flow mobile. This ground breaking approach surpasses conventional hydrodynamic methods, leading to speedier processing speeds, Extraordinary details quality, and increased sample throughput.

Since the cell line experienced Formerly been analyzed around the Attune CytPix Flow Cytometer, the researchers went again to the images and were being in the position to document the microbial infection not less than 5 times previously.

Agilent Benefit Assure guarantees you not less than 10 years of instrument use from the day you purchase. Or else, We're going to credit history you Along with the residual worth of the method toward an upgraded model.

What gave the impression to be a homogeneous populace is in fact far more various—an insight that ought to be deemed when interpreting outcomes.